Fig. 7: Cell clustering and functional annotation of tumor-infiltrating lymphocytes (TILs) in OS lesions. | Nature Communications

Fig. 7: Cell clustering and functional annotation of tumor-infiltrating lymphocytes (TILs) in OS lesions.

From: Single-cell RNA landscape of intratumoral heterogeneity and immunosuppressive microenvironment in advanced osteosarcoma

Fig. 7: Cell clustering and functional annotation of tumor-infiltrating lymphocytes (TILs) in OS lesions.

a t-SNE plot for TILs in OS lesions, and the cells were classified into seven subclusters. b The violin plots showing the normalized expression levels of 8 signature genes across the TIL subclusters. c Dot plots showing 14 signature genes among the TIL subclusters. The size of dots represents the proportion of cells expressing the particular marker, and the spectrum of color indicates the mean expression level of the markers (log1p transformed). d The t-SNE plot showing the expression profiles of the four selected well-known marker genes for exhausted T cells. e Heatmap of the gene sets of T-cell cytotoxicity, exhaustion, regulatory cytokines and receptors, naive T cells, and T-cell costimulation, based on GSVA enrichment analysis. f Blockade of TIGIT increases the CD3+ T cells mediated cellular cytotoxicity activities on U2OS and 143B cells derived from patients BC3 and BC16, but not for BC5 and BC6 (n = 3). Error bar: mean value ± SD. P values were determined by paired two-sided Student’s t-test. The source data for the relative T cellular cytotoxicity activities on OS cell lines (f) are provided in the Source Data file.

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