Fig. 5: BAH–PHD–CPL2 complex binds to the H3K27me3-marked genes to repress the transcriptional initiation and elongation of Pol II.

a ChIP-qPCR showing the fold enrichments of AIPP3, AIPP2, and PAIPP2 on the selected genes. The occupancy was first normalized to the internal control AtSN1, and shown are relative fold enrichments compared to Col-0. The data are means ± S.D. of three biological repeats. Unpaired one-tailed t test was performed and *p value < 0.01. b Snapshots of AIPP3-4Myc ChIP-seq showing the distribution of the AIPP3 protein at the selected genes. Note: the ChIP-qPCR and ChIP-seq results of AIPP3-4Myc were from independent samples. c, d The diagrams showing AIPP3 occupancies on aipp3-1 up-DEGs (b) and AIPP3-bound genes (c). e The diagrams showing the H3K27me3 (left) and H3K4me3 (right) density on AIPP3-bound genes. Random regions serve as negative controls. f A Venn diagram showing the overlap between AIPP3-bound genes, H3K27me3-enriched genes, and EMF1-bound genes.