Fig. 6: The H3K27me3-binding activity is essential for AIPP3-mediated repression of flowering and gene expression.

a The flowering phenotypes of the aipp3-1 mutant for its complementary lines when transformed with the wild-type AIPP3 genomic DNA, mutated AIPP3 in which the key amino acids for H3K27me3-binding were mutated. For the mutated transgene, two randomly selected transgenes were used for the analysis. The plants were grown under LD. b The column showing the numbers of rosette leaves (LDs) of different AIPP3 transgene plants upon flowering under the LD condition. Black horizontal lines represent the mean, and the error bars represent ±S.D. from the number of plants counted for each genotype. n = 15 per line. c Western blotting result showing the accumulation levels of AIPP3 proteins in different transgenes. Ponceaus staining serves as protein loading controls. d The relative mRNA levels of the selected target genes of the BAH–PHD–CPL2 complex in the wild-type, aipp3-1, and different AIPP3 transgene plants. Their levels are presented relative to Col-0. The data are the means ± S.D. of three biological repeats. Unpaired one-tailed t test was performed and *p value < 0.01. e ChIP-qPCR resulting showing AIPP3 occupancy at the selected target genes in AIPP3 and W170A transgenes. The data are the means ± S.D. of three biological repeats. Unpaired one-tailed t test was performed and *p value < 0.01. ns no significance.