Fig. 2: PS of VMH SF1 projections to the PVT inhibits food intake. | Nature Communications

Fig. 2: PS of VMH SF1 projections to the PVT inhibits food intake.

From: An excitatory ventromedial hypothalamus to paraventricular thalamus circuit that suppresses food intake

Fig. 2: PS of VMH SF1 projections to the PVT inhibits food intake.The alternative text for this image may have been generated using AI.

a Schematic illustration of viral transduction of VMH SF1 neurons with ChR2-eYFP in SF1-Cre mice and an optic fiber above PVT for PS. b, c Representative images of b1, b2 ChR2-eYFP-transduced SF1 neurons in VMH and c ChR2-positive SF1 projections in the PVT and the optic fiber track above PVT. d, e PS of ChR2-expressing SF1 projections in the PVT significantly reduced food intake in ChR2-transduced mice (n = 4 males and 4 females) but not in control eYFP-transduced mice (n = 3 males and 3 females): d Group data of food intake collected from fed mice in the LLP at the intervals of 30 min with blue laser off and on respectively (eYFP vs ChR21st on, p = 0.0121; eYFP vs ChR22nd On, p = 0.0021); e group data of refeeding collected from 24 h food-deprived mice in the ELP at the intervals of 1 h before, during and after blue laser on (eYFP1st off vs On, p = 0.7117; eYFP On vs 2nd off, p = 0.4985; ChR21st off vs On, p = 0.001; ChR2 On vs 2nd off, p < 0.0001). Two-tailed Student’s t-test; data represent mean ± s.e.m.; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bars, 200 μm for b1, 100 μm for b2 and c. DMH dorsomedial hypothalamus, PVT paraventricular thalamus, V3 third ventricle, VMH ventromedial hypothalamus, PS photostimulation.

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