Fig. 2: Controlled, homogeneous uptake of sfGFP-His into NTA-functionalized protocells.
From: Programmed spatial organization of biomacromolecules into discrete, coacervate-based protocells
a False-colored confocal micrographs of protocells show the specific uptake of sfGFP-His (green) by Ni-NTA-Am. The membrane is stained with Nile Red (purple), scale bar: 10 µm. Uncropped images are to be found in Supplementary Fig. 7. b Flow cytometry analysis of sfGFP-His loaded protocells. For clarity of the graph, a 10th of all data points collected is shown. Gating parameters and population statistics are reported in Supplementary Fig. 8. c Box plot analysis of the measured concentration of sfGFP-His measured inside the coacervate interior with confocal microscopy. Reference curve Supplementary Fig. 9. n = 69.
