Fig. 5: Lateral opening mutants of TodX and CymD have impaired MAH uptake.
From: Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion
a Representative growth of Tn7-complemented PpF1-3 strains on toluene (left panel) and p-cymene vapour in the presence of 0.5% arabinose. Growths shown are representative of 2 or 3 plates. b Corresponding maximum colony sizes from (a), taken from two different plates (mean ± S.D; n = 6–10). c Representative growth of pHERD30-complemented PpF1-3 strains on toluene (left panel) and p-cymene vapour in the presence of 0.1% arabinose. Growths shown are representative of 2 or 3 plates. d Corresponding maximum colony sizes from c, taken from 2 different plates (mean ± S.D; n = 6–10). e Coomassie-stained SDS-PAGE gel of pHERD30-complemented PpF1-3 strains after IMAC. lanes 1/7, empty plasmid; lanes 2/8, CymD wild type; lanes 3/9, CymD ΔS2; lanes 4/10, CymD ΔS3; lanes 5/11, CymD ΔS2S3; lanes 6/12 CymD G105A. Arrows indicate approximate positions of CymD variants before (lanes 1–6) and after boiling (lanes 7–12). As is the case for many OMPs, CymD remains partially folded in SDS without boiling, leading to faster migration in the gel and a lower apparent molecular weight (~31 kD compared to ~48 kD after boiling). Molecular weight markers are indicated on the left. The gel picture shown is representative of two independent expression analyses. Source data for colony sizes are provided in the Source Data file.
