Fig. 4: 8-OHG induces macrophage migration and activity.

a, b Levels of 4-HNE or 8-OHG in the pancreas (a) or serum (b) in Pdx1-Cre;K-Ras^G12D/+ (KC) or Pdx1-Cre;K-Ras^G12D/+;Gpx4^−/− (KCG) mice with or without liproxstatin-1 (Lip1) or clophosome (Clop) treatment (n = 3 mice/group; two-way ANOVA with Tukey’s multiple comparisons test). c–e Mouse bone marrow-derived macrophages (BMDMs) or human blood monocyte-derived macrophages (HPBMs) were treated with 4-HNE (500 ng/ml) or 8-OHG (500 ng/ml) or guanosine (“G”, 500 ng/ml) for 24 and 48 h. The cell migration (c) and mRNA levels or release of Il6 (d) and Nos2 (e) were assayed (n = 3; two-way ANOVA with Tukey’s multiple comparisons test). f Co-localization of 8-OHG DNA and CGAS in tumors of KC and KCG mice at 3 months of age (n = 5 mice/group; one-tailed t test). g Expression of CGAS and TMEM173 in KC mice at 3–12 months of age (n = 5 mice/group; two-way ANOVA with Tukey’s multiple comparisons test). h–j BMDMs from wild-type (WT), Tmem173^−/−, or Tlr9^−/− mice were treated with 8-OHG (500 ng/ml) for 24 and 48 h. The cell migration (h) and mRNA levels of Il6 (i) and Nos2 (j) were assayed (n = 3; two-way ANOVA with Tukey’s multiple comparisons test). Data in a–j are presented as mean ± SD. Data are from two or three independent experiments.