Fig. 1: DMV cholinergic neurons receive POMC input from the ARC.
a Schematic diagram of the experimental configuration. AAV1-FLEX-GFPsm viruses were injected into the ARC of POMC-Cre mice (left panel). Expression of GFP-positive cells was clearly visible in in the ARC (right panel). Scale bar: 100 µm. VMH ventromedial hypothalamus. b Images of confocal fluorescence microscopy showing double immunostaining with anti-GFP (green, left panel) and anti-ChAT (red, middle panel) antibodies. GFP-positive fibers were visible in the DMV (right panel), representing DMV cholinergic neurons received POMC input from the ARC. Scale bar: 50 µm. CC central canal. c, d Representative traces showing reduced DMV cholinergic neuron activity in response to treatment with MTII (100 nM) in the absence of synaptic blockers (c). Plots showing individual data as to mean frequency of action potentials before and after treatment with MTII (d) (mean frequency: 1.3 ± 0.3 Hz vs. 0.3 ± 0.1 Hz, n = 7 out of ten neurons, two-tailed t test, *p = 0.02; Vm: −45.6 ± 1.8 mV vs. −45.8 ± 2.2 mV, two-tailed t test, p = 0.9). All data are shown as mean ± SEM. Source data are provided as a Source Data file.
