Fig. 6: Insulin-induced hypoglycemia increases calcium signal in ARC POMC neurons. | Nature Communications

Fig. 6: Insulin-induced hypoglycemia increases calcium signal in ARC POMC neurons.

From: Optogenetic stimulation of the liver-projecting melanocortinergic pathway promotes hepatic glucose production

Fig. 6

a Schematic illustration of the experimental configuration. We injected AAV5-Syn-FLEX-GCamp6s viruses into the ARC of POMC-Cre mice (left panel). Right panel shows the expression of GFP in ARC POMC neurons in mice injected with these viruses into the ARC. Scale bar: 100 µm, 3V, third ventricle. b Average of normalized ARC POMC neuron calcium signal in mice treated with saline and insulin (2 U/kg) (n = 6 mice, respectively). Insulin-induced hypoglycemia caused an increase in calcium signals approximately 1 h post i.p. insulin injection. Dark blue lines represent error bars. Source data are provided as a Source Data file. c Summary plot showing changes in normalized ARC POMC neurons calcium signal over time in mice treated with saline and insulin (significance assessed using a two-way ANOVA followed by Sidak multiple comparisons test, 90 min, **p = 0.006). All data are shown as mean ± SEM. Source data are provided as a Source Data file.

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