Fig. 2: DNA-damaging treatments induce the centrosome clustering protein KIFC1. | Nature Communications

Fig. 2: DNA-damaging treatments induce the centrosome clustering protein KIFC1.

From: The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation

Fig. 2

a KIFC1 protein expression in PDX models after treatment with PBS, etoposide, or cisplatin. Data are representative of stained tumor tissues (scale bar, 50 μm) with quantitative analysis. Two-tailed t test p values: p = 0.04721 (BRPF008, Eto), 0.0339 (008, Cis), 0.0350 (212, Eto), 0.0390 (212, Cis), 0.0341 (232, Eto), 0.0202 (232, Cis), 0.0263 (280, Eto), and 0.0130 (280, Cis). (b, c) MDA-MB-231 cells (b) or indicated cells (c) were treated with irradiation (IR), ultraviolet (UV) light, etoposide (10 μM), cisplatin (10 μM), oxaliplatin (40 μM), mitomycin C (2 μM), estramustine (20 μM), epirubicin (0.5 μM), gemcitabine (4 μM), bleomycin (10 μM), or cyclophosphamide (CTX, 10 mM) for 15 h. Cell lysates were immunoblotted with antibodies against KIFC1, γH2AX, or β-actin (as the internal standard). d MDA-MB-231 xenograft tumors were treated with etoposide, IR, or cisplatin as described in the Methods section. Data are representative of stained tumor tissues (scale bar, 50 μm) with quantitative analysis. Three tumors were included in each group. Two-tailed t test p values: p = 0.0424 (Eto), 0.0345 (IR), and 0.0415 (Cis). Data represent the mean ± SD of three independent experiments (a, d). *p < 0.05, **p < 0.01, Source data are provided as a Source Data file.

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