Fig. 4: Anti-senescent effects of the top four compounds.
From: Anti-senescent drug screening by deep learning-based morphology senescence scoring
a Representative images of SA-β-gal activity in senescent HUVECs treated with the top four compounds: terreic acid, PD-98059, daidzein, and Y-27632. Scale bar: 100 μm. Data are representative of two independent experiments. b Percentage of SA-β-gal-positive cells (n = 12 images over two independent experiments). c Western blotting of P21, P53, Ser15 phosphorylation of P53, and P16INK4a. The top four compounds were added to HUVECs, and cellular senescence was induced by H2O2. GAPDH was used as an internal control. Data are representative of two independent experiments. d A heatmap shows the top 10 genes selected by differential gene expression analysis among all four compounds and control. The genes designated in red are associated with NFκB function. e GSEA of genes associated with the inflammatory response. f GSEA of genes associated with TNFα signalling via NFκB. g GO analysis, categorised as biological process, of genes upregulated in HUVECs treated with terreic acid compared with control. h, i GSEA of genes associated with h positive regulation of ATPase activity and i oxidative phosphorylation. NES normalised enrichment score. Data are shown as mean ± s.e.m. p values by two-sided Student’s t-test.
