Fig. 1: TBPL2 does not assemble in a TFIID-like complex in growing oocytes.
From: TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage
a Anti-TBPL2 immunoprecipitation followed by mass spectrometry (IP-MS) analysis from three biological replicates of mouse ovarian whole-cell extracts (WCE). The colour code for the different proteins or complexes is indicated on the right. NSAF; normalised spectral abundance factor. b Anti-TBP IP-MS from ovarian WCE (three technical triplicates). The colour code is the same as in (a). c–f Sequential IP-MS experiment from ovarian WCE (three technical triplicates). The strategy of the sequential immunoprecipitation (c), anti-TAF7 IP-MS (d), followed by an anti-TAF10 IP-MS (e) and then an anti-TBPL2 IP-MS (f). The colour code is the same as in (a). g, h Representative views of haematoxylin and eosin-stained ovaries sections from control (g) and oocyte-specific Taf7 mutant (Tg(Zp3-Cre/+);Taf7flox/∆, h) ovaries (analyses of three sections from three biological replicates). The presence of antral follicles is indicated by an asterisk. Scale bars: 500 µm. In (a, b, d–f) grey dots indicate replicates and error bars, +/− standard error of the mean.
