Fig. 2: Zinc-binding groups allow probing HDACs in microarray format. | Nature Communications

Fig. 2: Zinc-binding groups allow probing HDACs in microarray format.

From: Hydroxamic acid-modified peptide microarrays for profiling isozyme-selective interactions and inhibition of histone deacetylases

Fig. 2: Zinc-binding groups allow probing HDACs in microarray format.

a Approved drugs and examples of candidates in clinical trials that target class I HDACs31. Shared features include a cap group, a hydrophobic linker region, and zinc-binding group. b Display and probing of modified 15-mer histone peptides for capture of HDAC3 (10 nM) on microarray slides. Incorporation of a 2-aminosuberic acid hydroxamic acid derivative (Asuha) or a 2-aminosuberic acid o-aminoanilide derivative (Asuapa) but not the carboxylic acid (Asu) allow probing of HDAC binding in microarray format (n = 2 independent experiments). Source data are provided as a Source Data file. *Saturated chemiluminescence signal.

Back to article page