Fig. 1: Autophagy is crucial for maintaining amino acid pools under nitrogen starvation. | Nature Communications

Fig. 1: Autophagy is crucial for maintaining amino acid pools under nitrogen starvation.

From: Autophagy sustains glutamate and aspartate synthesis in Saccharomyces cerevisiae during nitrogen starvation

Fig. 1

a Schematic of the experimental design. WT (ATG1) and atg1Δ cells were grown in synthetic defined medium (SD) and starved for nitrogen (SD-N) for varying times. Metabolites were extracted and analyzed by LC-MS/MS. b Changes of amino acids in nitrogen-starved WT and atg1Δ cells. Three independent replicates for pro and asn, and six for the remaining amino acids. Absolute concentration was calculated based on Supplementary Fig. 3b by comparing different time points of WT and atg1Δ cells to WT grown in SD. WT and atg1Δ cells were always processed and analyzed together and therefore their abundances are directly comparable. hs: homoserine. P values were calculated using unpaired two-sided Student’s t test assuming equal variances. Data are presented as mean ± standard deviation.

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