Fig. 4: Characterization of rapamycin-induced autophagy in the presence of nitrogen. | Nature Communications

Fig. 4: Characterization of rapamycin-induced autophagy in the presence of nitrogen.

From: Autophagy sustains glutamate and aspartate synthesis in Saccharomyces cerevisiae during nitrogen starvation

Fig. 4

a Rapamycin treatment induces autophagy in the presence of nitrogen. Three independent replicates. All samples were normalized to WT cells grown in SD. b Changes of glutamate and aspartate in rapamycin-treated WT (ATG1) and atg1Δ cells in the presence of nitrogen. Three independent replicates. c Autophagy deficiency renders yeast cells more recalcitrant to growth inhibition by rapamycin. Cells were grown in SD to log phase, and washed and resuspended in prewarmed SD + rapamycin with a starting OD600 ~0.1. Growth was monitored by measuring OD600 at indicated time intervals. Three independent replicates. P values were calculated using unpaired two-sided Student’s t test assuming equal variances. Data are presented as mean ± standard deviation.

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