Fig. 5: Loss of the triple GEFs results in a downregulation of junctional RAC1 activity.
From: A RAC-GEF network critical for early intestinal tumourigenesis
a Immunoprecipitation of Active RAC1 from intestinal epithelium, blotted with total RAC1 and the corresponding scoring. n = 3 biologically independent samples for Vil-CreERT2 Apcfl/fl (APC) and Vil-CreERT2 Apcfl/fl, Vav2−/−, Vav3−/− Tiam1−/− (APC V2V3T). *p = 0.00799 as determined by a one-tailed Mann–Whitney test. Data are presented as mean values ±SD. b FLIM-FRET analysis of organoids day 3 post isolation from Vil-CreERT2 Apcfl/fl (APC) and Vil-CreERT2 Apcfl/fl Vav2−/−, Vav3−/−, Tiam1−/− (APC V2V3T) Scale bar represents 20 μm. c Quantification of FLIM-FRET analysis at cell–cell contacts. N = 3 biologically independent cell lines derived from independent mice for each genotype. *p = 0.0426 as determined a two-tailed unpaired T-test. Data are presented as mean values ±SD.
