Fig. 2: Site-specific incorporation of SM60 in EGFP and subsequent conversion to citrulline in HEK293T cells.
From: Site-specific incorporation of citrulline into proteins in mammalian cells
a EGFP-39-TAG reporter expression by EcLeuRS-tRNACUAEcLeu pair in HEK293T cells in the presence of SM60 indicated by the fluorescence of EGFP. b Quantification of EGFP-39-TAG reporter expression efficiency in the presence of an increasing concentration of SM60. n = 3 independent experiments, data are presented as mean value ± SD. The solid circles represent data points for biologically independent replicates. Source data are provided as a Source Data file. c Coomassie stain of purified EGFP containing SM60. Full gel is given in Supplementary Fig. 7. d Deconvoluted mass spectrum of EGFP before and after 365 nm UV irradiation (1 min), indicating the presence of SM60 and citrulline, respectively, at position 39. Non-deconvoluted spectra are given in Supplementary Figs. 8 and 9.
