Fig. 3: Severe neuron damage in ELT-EAE mice. | Nature Communications

Fig. 3: Severe neuron damage in ELT-EAE mice.

From: Early-life-trauma triggers interferon-β resistance and neurodegeneration in a multiple sclerosis model via downregulated β1-adrenergic signaling

Fig. 3: Severe neuron damage in ELT-EAE mice.

a, b Golgi–Cox stained lumbar spinal cord sections of EAE mice isolated at 30 dpi (a) with quantification of area stained/per field (b) (Cont naïve: n = 3, ELT naïve: n = 4, Cont EAE: n = 3, ELT-EAE: n = 3). c, d CRSR imaging of Golgi–Cox-stained dendrites in lumbar spinal cord ventral horn region (c) with quantification of dendritic spine density at 30 dpi (d) (n = 3 animals/group, 60 dendrites/group). e FluoroJade-C-stained lumbar spinal cord sections of EAE mice isolated at 20 dpi with quantification of area stained/per field (Cont EAE: n = 7, ELT-EAE: n = 4). f ChAT+ neuron-stained lumbar spinal cord sections of EAE mice isolated at 30 dpi (n = 3 per group). g Representative image of Iba1+ cells in spinal cord ventral horn at 30 dpi. Representative image was randomly selected from pooled images from two independent staining experiments. h Iba1+ cell count categorized by soma size in the lumbar spinal cord ventral horn (L5–6) at 30 dpi (n = 3 per group). Each dot represents averaged data per animal. Data are represented as mean ± SEM. Two-tailed Student’s t-test, *P < 0.05. Exact P values for asterisks (from left to right): b 0.0005, 0.0350; d 0.0382, 0.0102; e 0.0007; f 0.0007, 0.0072; h 0.0204.

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