Fig. 4: Oligomeric interfaces on inflammasome CARD domains.
From: Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8
a Left panel: side view of electrostatic potential distribution of a segment of NLRP1-CARD filament (red, −10 C/m2; blue, 10 C/m2; generated in Chimera). Right panel: electrostatic potential map of a NLRP1-CARD monomer in the same orientation as the filamentous oligomer. b Left panel: side view of electrostatic potential distribution of a segment of CARD8-CARD filament (red, −10 C/m2; blue, 10 C/m2). Right panel: electrostatic potential map of a CARD8-CARD monomer in the same orientation. c Atomic model cartoon and the directly observed density map of a NLRP1-CARD dimer within the filamentous complex, centered at the Type II junction interface. The four residues, His1452-His1454 on the Type IIb interface and the Ser1435-Asp1437 on the Type IIa interface were highlighted and shown in the “stick” mode. d Atomic model cartoon and the directly observed density map of CARD8-CARD dimer in the filament, centered at the Type II junction interface. The four residues, Asp525–Tyr527 on the Type IIb interface and the Lys508–Leu512 on the Type IIa interface were highlighted and shown in the “stick” mode. e Electrostatic potential map of the top view (Type IIb interface, highlighted in yellow dashed area) of the NLRP1-CARD filament. f Electrostatic potential map of the top view (type IIb interface, highlighted in yellow dashed area) of CARD8-CARD filament. g Electrostatic potential map of the bottom (type IIa highlighted in yellow dashed area) and top (type IIb highlighted in yellow dashed area) views of the ASC-CARD filament. h Electrostatic potential map of the bottom (type IIa highlighted in yellow dashed area) and top (type IIb highlighted in yellow dashed area) views of the CASP1-CARD filament.
