Fig. 8: Reactivation of QKI5 in hESCs-QKIdel rescues myofibrillogenesis. | Nature Communications

Fig. 8: Reactivation of QKI5 in hESCs-QKIdel rescues myofibrillogenesis.

From: QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function

Fig. 8: Reactivation of QKI5 in hESCs-QKIdel rescues myofibrillogenesis.The alternative text for this image may have been generated using AI.

Inducible QKI5, QKI6, and QKI7 expression cells are established in H1-8 lines (H1-8-QKI5ind, H1-8-QKI6ind, H1-8-QKI7ind). Representative images of immunofluorescence staining of TNNT2 and ACTN2 in H1-8-QKI5ind, H1-8-QKI6ind, H1-8-QKI7ind cardiomyocytes with or without doxycycline induction. Myofibrillar structures are recovered in doxycycline-induced H1-8-QKI5ind cardiomyocytes (Day-15) and ACTN2 expression is also recovered. However, doxycycline-induced H1-8-QKI6ind and H1-8-QKI7ind cardiomyocytes fail to recover ACTN2 expression and generate normal myofibrillar structures (Day-15). Scale bar: 25 μm. All experiments are independently repeated three times with three different sets of cell samples to confirm the reproducibility of the findings.

Back to article page