Fig. 6: Slide isoform sequencing (sl-ISO-Seq) confirms spatial localization of splicing changes.
From: A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain
a Spatial localization of hippocampal single-cell excitatory neuron subtypes using gene-expression similarities to HIPP short reads. Color of spot indicates percentage of transcripts corresponding to indicated cell type. b Spatial localization of cortical excitatory neuron subtypes using gene-expression similarities to PFC short reads. Color of spot indicates percentage of transcripts corresponding to indicated cell type. c Scatter plot of the ΔΨ between HIPP and PFC from the single-cell data, and the ΔΨ between HIPP and cortex from 10X Visium spatial data (r2 = 0.6). d Spatial distribution of alternatively spliced exons in Clta and Pkm genes in the hippocampal and choroid plexus regions. Color of spot indicates Ψ values for each exon. e Spatial distribution of two mutually exclusive, alternatively spliced exons in Snap25 gene across the whole slide. Color of spot indicates Ψ values for each exon.
