Fig. 2: iHUCOs demonstrate aberrant adherens junction formation in the epithelium.
From: Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
a, c Representative IHC for β-catenin and E-cadherin, demonstrating the difference in cellular localization between iHNOs and iHUCOs, as well as their matched primary tissues. b, d Violin plots of the percentages of cells expressing β-catenin and E-cadherin in the organoids and primary tissues as distinguished by the cellular compartment: plasma membrane only (Mem) or membrane extended to cytoplasm and nucleus (Cyt + Nuc). e Representative IHC for RhoA, demonstrating increased cytoplasmic and membrane expression of RhoA in iHUCO vs. iHNO, and UC vs. normal primary tissues. f Violin plot indicating the percentage of cells positive for RhoA according to cellular localization in the organoids, plasma membrane only (Mem), or cytoplasm (Cyt), and the primary tissues. g Representative dual IF staining for E-cadherin (green) and β-catenin (red) in iHNO and iHUCO. h Violin plots of cells in iHNOs and iHUCOs co-expressing E-cadherin and β-catenin proteins extended to cytoplasm and nucleus (Cyt + Nuc) or in plasma membrane only (Mem). All nuclei are stained with DAPI (blue). An unpaired, non-parametric, two-sided Mann–Whitney U test was used for all comparisons. IF scale bar = 25 μm, IHC scale bar = 40 μm. n = 5 iHNOs (blue) and n = 6 iHUCOs (orange). Source data are provided as a Source Data file.
