Fig. 6: High-resolution transcriptomic analysis of iHUCOs highlights their parental signature.
From: Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
a UMAP of the predicted clusters in iHNOs and iHUCOs (n = 3 each). Different colors are used to distinguish clusters. Inset, pink are iHUCO clusters, while turquoise are iHNO clusters. b Top expressed genes of the predicted clusters in iHNOs and iHUCOs shown as a marker plot. The color and size of dots correlate with mean expression level and fraction of cells per cluster expressing each gene, respectively. Cluster number is colored to match parental cluster. Pink number indicates UC fibroblast origin while turquoise indicates normal colon fibroblast clusters. c Reactome pathway analysis based on the DEGs in the epithelial and stromal compartments of iHUCOs vs. iHNOs, shown as bar graphs for the selected highly enriched and significant terms. n = 30,089 cells for iHNO and n = 43.420 cells for iHUCO.
