Fig. 7: Single nucleus profile of iHNOs corresponds with their fibroblasts of origin.
From: Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
a Proportion plots to demonstrate the identified sub-populations in iHNO epithelium and mesenchyme. Cycling TA = cycling transient amplifying cells; Myo FB = myofibroblasts; PC venules = pericyte venules. b Proportion plots illustrating sub-populations per cell type in iHUCOs. Cycling TA = cycling transient amplifying cells; I. enterocyte = immature enterocytes; I. goblet = immature goblet cells; Myo FB = myofibroblasts; cycling B = cycling B cells; NK = natural killer cells. c–f Representative IHC for HLA-A-expressing and Limch1-expressing epithelia in iHNO vs. iHUCO with violin plots showing a significant increase in expression of both proteins in iHUCOs compared with iHNOs. The unpaired, non-parametric two-sided Mann–Whitney U test was used to test for the difference in percentages between iHNO and iHUCO. Scale bar = 40 μm. n = 5 iHNOs (blue) and n = 6 iHUCOs (orange). g Representative cytokine arrays of normal and UC parental fibroblasts and organoid-derived mesenchyme with the quantification of three chemokines are increased in UC vs. normal: GRO-α (green), GRO − α + β + γ (blue), and CXCL8 (orange). The median value is indicated in the center of the box plot. The whiskers above and below the plot represent 1 standard deviation (SD) above and below the mean of the data, respectively. Source data are provided as a Source Data file.
