Fig. 9: Repertaxin attenuates in vivo progression of the colitic phenotype in iHUCOs.
From: Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
a Schematic representation of the in vivo repertaxin treatment study; spheroids encapsulated in TS-HA hydrogel were implanted subcutaneously in the dorsal flanks of immunocompromised NSG mice, which then received daily injections of repertaxin or PBS (control) for 21 days. b, c Representative dual IF staining for CXCR1 (red) and CXCL8 (green) in control (Ctrl; no treatment) and repertaxin-treated (20 mg/kg) implanted organoids. d, e Percentage distribution of cells positive for CXCR1, CXCL8 and both (overlap) in the epithelium and mesenchyme of normal and UC organoids in the presence or absence of repertaxin. f–g Representative H&E of control and repertaxin-treated organoids collected at 21 days. h, i Average organoid diameter and percentage of each epithelial structure type in the presence or absence of repertaxin. The unpaired, non-parametric, two-sided Mann–Whitney U test was used to test whether the percentage of cells expressing the specific morphology differed. The median value is indicated in the center of the box plot. The whiskers above and below the plot represent 1 standard deviation (SD) above and below the mean of the data, respectively. j, k, n, o Representative IHC for β-catenin and E-cadherin in control and repertaxin-treated organoids revealing the altered cellular localization of both proteins in iHN and iHUC organoids by repertaxin treatment. l, m, p, q Distribution of the epithelial cells positive for β-catenin and E-cadherin with or without repertaxin treatment. Results reported for the plasma membrane only (Mem) or membrane extended to cytoplasm and nucleus (Cyt + Nuc). r, s Representative IHC for RhoA in normal and UC organoids, confirming the effect of repertaxin on RhoA sub-cellular localization. t, u Percentages of cells expressing RhoA in control and repertaxin-treated organoids according to cellular compartment: plasma membrane only (Mem) or cytoplasm (Cyt). The unpaired, non-parametric, two-sided Mann–Whitney U test was used to test for the differences between all enumerated observations. IHC scale bar = 40 μm. IF scale bar = 25 μm. n = 3 each. Source data are provided as a Source Data file.
