Fig. 4: Plasticity of SufB2-induced +1 frameshifting.
From: Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA
a fMP formation as a function of time upon delivery of the G37C variant of G37-state SufB2-TC to the A site of a 70S IC, allowing nucleotides 34–36 to pair with a CCC-C motif at the A site. b fMP formation as a function of time upon delivery of the G34C variant of G37-state SufB2-TC to the A site of a 70S IC, allowing nucleotides 35–37 to pair with a CCC-C motif. c–f Results of fMPV formation assays in which SufB2-TC is delivered to an A site programmed with a quadruplet codon at the 2nd position and sequences of the SufB2 anticodon loop and/or quadruplet codon are varied. Yields of fMPV formation represent +1 frameshifting during translocation of SufB2 from the A site to the P site. Possible +1-frame anticodon-codon pairing schemes of SufB2 during translocation: c G37-state SufB2 capable of frameshifting at a CCC-C motif via quadruplet pairing and/or triplet slippage, d G37C variant of G37-state SufB2 capable of frameshifting at a GCC-C motif via quadruplet pairing and/or triplet slippage, e m1G37-state SufB2 capable of frameshifting at a CCC-C motif via only triplet slippage, and f G37C variant of G37-state SufB2 capable of frameshifting at a CCC-C motif via only triplet slippage. In a, b the bars in the graphs are SD of three (n = 3) independent experiments, and the data are presented as mean values ± SD. ∆t: a time interval.
