Fig. 5: SufB2 shifts to the +1-frame during translocation.

a Relative fMPV and fMPR formation as a function of time upon rapid delivery of EF-G and an equimolar mixture of G37-state SufB2-, tRNA^Val-, and tRNA^Arg-TCs to 70S ICs carrying a CCC-C motif in the A site. b Relative fMPV and fMPR formation as a function of time when a defined time interval is introduced between delivery of G37-state SufB2-TC and EF-G and delivery of an equimolar mixture of tRNA^Arg- and tRNA^Val-TCs. c Relative fMPV and fMPR formation after reacting fMP-POST complexes with a mixture of tRNA^Val- and tRNA^Arg-TCs based on the time courses in Supplementary Fig. 2d–f. d fMPV formation as a function of time upon rapid delivery of tRNA^Val-TC to an fMP-POST complex carrying a CCC-N motif in the A site. e Relative fMPV and fMPS formation as a function of time upon rapid delivery of an equimolar mixture of tRNA^Val- and tRNA^Ser-TCs to an fMP-POST complex carrying a CCC-A motif in the A site. In a–e the bars are SD of 3 (n = 3) independent experiments and the data are presented as mean values ± SD. Arg: arginyl-tRNA^Arg; Val: valyl-tRNA^Val.