Fig. 3: Schematic of key steps involved in modular RLPs synthesis.
From: Resilin-mimetics as a smart biomaterial platform for biomedical applications
The domain selection step involves the selection of peptide domains of desired functions, the design step involves the arrangement of the intended amino acid sequence and encoding genetic sequence, the incorporation step involves the construction of a genetic vector and their transfection into a host organism, the expression involves induced expression of the intended modular RLPs in host organism, and purification step involves extraction of pure polypeptides by chromatographic and/or non-chromatographic (cold-coacervation) methods.
