Table 1 Summary of modular RLPs synthesized and their reported structure–property transformations.
From: Resilin-mimetics as a smart biomaterial platform for biomedical applications
Modular RLPsa | Secondary domains | Structure–property transformations | Applications | Ref. |
|---|---|---|---|---|
RLP12-RGD2-MMP-HBD, RLP12-RGD, RLP12-HBD, RLP12-MMP | Cell-binding sequence (RGD), matrix metalloproteinase cleavable peptide (MMP), heparin-binding domain (HBD) | Increase in cellular adhesion, biodegradation and metabolic activity (with no inflammatory response), and mechanical property | Tissue engineering | |
REC or RLP4-ELP7-CLP2 | Elastin-like polypeptide (ELP), collagen-like polypeptide (CLP) | Self-assembled network of flexible often aligned fibres (different to that of the twisted rope-helical structure) at physiological temperature | Tissue engineering | |
AnX-EGFP (where X = 4, 8, 16 and 32) | Enhanced green fluorescence protein (EGFP) | Autofluorescence. | Bioimaging | |
RZ10-RGD, RZ10-BMP2, RZ10-QK | Cell-binding sequence (RGD), bone morphogenetic peptide (BMP), vascular endothelial growth factor-mimicking domain (QK) | Increase in cellular adhesion and differentiation, and mechanical property | Tissue engineering | |
RLPn-SLPn (where n = 1, 4 and 8) | Silk-like polypeptide (SLP) | Spherical micelle (SLP core) to nanofibril to large coalescence with increase in temperature. Upshift in UCST and stiffness of hydrogel with increase in RLP length. Increase in cellular adhesion and mechanical property | Drug delivery, tissue engineering | |
(RLP)n-(X-ELP)Y (where n = 20, 40, 60, 80 and 100; Y = 40, 80 and 160; X is amino acid residue S, A/G (equal content) and V | Elastin-like polypeptide (ELP) | Spherical micelle (RLP core) to soluble chains to coalescence with increase in temperature. Spherical to cylindrical morphology transition with increase in RLP chain length and hydrophobicity of the ELP | Drug delivery, tissue engineering | |
CBM2-RLP-HFBI, CBM-RLP-CBM | Cellulose-binding module (CBM), hydrophobin protein domain (HFBI) | Increase in coacervate size with increase in temperature and pH. Selective adhesion and self-assembly on different surfaces | Tissue engineering, drug delivery, biosensor | |
GB1-RLP4, GB1-RLP-(GB1)5-RLP-(GB1)4-RLP | Artificial elastomeric protein (GB1) | Increase in mechanical property | Tissue engineering | |
RLP12-LCD5 | Lysine crosslinking domain (LCD) | Increase in lysine crosslinking site and mechanical property | Tissue engineering |
