Fig. 5: CTCF and RAD21 may be responsible for mediating enhancer–promoter interactions at MYC and BCL2.

a Capture-C and ChIP-seq for BRD4, CTCF and RAD21 at the MYC gene and enhancer region. Capture-C was conducted using the MYC promoter as the viewpoint, indicated by a vertical gray bar, mean of three biological replicates. Orientation of CTCF motifs at peaks is indicated by triangles. Locations of primers used for CTCF/RAD21 ChIP-qPCR (see Supplementary Fig. 5) are shown at the bottom of the figure. b Capture-C and ChIP-seq data at BCL2, as in a. c Capture-C traces from untreated cells (black line; mean of three replicates) and ChIP-seq for CTCF (blue) and RAD21 (pink). Vertical gray bar indicates the capture point for each gene. Orientation of CTCF motifs at peaks is indicated by triangles. Scale bars show 100 kb. Pink shading highlights promoter-interacting regions that overlap with CTCF/RAD21 peaks (visually determined). d Left: Capture-C profile from the MYC promoter showing the MYC enhancer in SEM cells with AID-tagged CTCF, either untreated (purple line) or treated with indole-3-acetic acid (IAA) for 48 h (blue line), which targets CTCF for degradation. Data are replotted from²², mean of two independent clones. Right: Capture-C profile from the MYC promoter showing the MYC enhancer in SEM cells treated with DMSO (purple line) or AT1 (green line) for 24 h, mean of three biological replicates. CTCF (blue) and RAD21 (pink) ChIP-seq tracks and CTCF motif orientations (triangles) are shown.