Table 1 Binding affinities between the spike protein (peaks 2 and 3) samples and hAPN measured using SPR.

From: Cryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changes

Spike protein

Kon (×104 M−1 s−1)

Koff (×10−3 s−1)

KD (nM)

Peak 2

3.18 ± 0.001

1.15 ± 0.002

36.3 ± 0.071

Peak 3

7.15 ± 0.001

0.485 ± 0.007

6.78 ± 0.105

  1. Values after ± correspond to the residual standard deviation. Each experiment was repeated independently twice with similar results.
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