Fig. 2: Cyclic voltammetry of an extended enzyme cascade trapped in electrode nanopores. | Nature Communications

Fig. 2: Cyclic voltammetry of an extended enzyme cascade trapped in electrode nanopores.

From: The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades

Fig. 2

a Scheme of the nanoconfined cascade driving reductive amination/carboxylation of pyruvate to aspartate in either direction through bidirectional electrocatalytic recycling of NADP(H) by the transducer enzyme FNR. CA carbonic anhydrase; FNR ferredoxin-NADP+-reductase; ME malic enzyme; FumC fumarase; AspA aspartate-amino-lyase. b Cyclic voltammetry (25 °C, pH 7.5, 1 mV s−1) of the 5-enzyme cascade (0.1 CA/1 FNR/5 ME/1 FumC/1 AspA) in buffer 0.05 M HEPES, 20 mM pyruvate, 0.1 M KHCO3, 0.1 M NH4Cl, 4 mM MgCl2, 1 mM MnCl2. Gray: blank, no cofactor present. Red: injection of NADP+ (to 20 µM). Blue: injection of aspartate (to 20 mM).

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