Table 1 Results of downstream experiments for aspartate synthesis starting from pyruvate (20 mM) using 20 and 5 µM NADP+.

From: The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades

Expt

Substrates (mmol)

Products (mmol)

Parameters

 

Pyruvate

NADP+

Malate

Fumarate

Aspartate

Time

TTN

Conversion

From coulometry

1

0.09

9 × 10−5

0.0065

0.0003

0.0306

First 20-h

340

34%

0.036

0.09

9 × 10−5

0.006

0.0001

0.0216

Second 20 h

240

24%

0.039

2

0.09

2.25 × 10−5

0.0035

0.001

0.0211

40 h

936

23.4%

0.029

  1. Current traces are shown in Supplementary Fig. S4 (experiment 1) and Supplementary Fig. S6 (experiment 2).  Volume in all cases = 4.5 mL.
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