Table 2 Kinetic data for ME, FumC, and AspA measured by conventional steady-state solution assays.

k_cat (s⁻¹)

17 ± 1.1

220 ± 54

503 ± 33

34 ± 3.1

220 ± 29

359 ± 15

K_M (mM)

8.6 ± 2.2

1.5 ± 0.11

0.19 ± 0.03

0.7 ± 0.22

0.6 ± 0.2

9 ± 1.6

k_cat/K_m(M⁻¹ s⁻¹)

2.0 × 10³

1.47 × 10⁵

2.65 × 10⁶

4.86 × 10⁴

3.67 × 10⁵

4.0 × 10⁵

τ (1/k_cat, ms)

58

4.5

2

29

4.5

2.7