Fig. 2: FLIM analysis of nuclear DNA in live U2OS cells stained with DAOTA-M2 (20 µM, 24 h).
From: Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
a Fluorescence intensity image recorded at 512 × 512 resolution (λex = 477 nm, λem = 550–700 nm), red lines represent the nuclear segmentation used for the FLIM analysis. b FLIM map from a, displayed between average lifetime (τw) 9 (red) and 13 (blue) ns. c 2D correlation of the average nuclear intensity against the average nuclear lifetimes (blue dots). d Zoomed-in FLIM map of a single nucleus – colours represent lifetimes as defined by the colour gradient bar between 9 (red) and 13 (blue) ns. e Histogram of fluorescence lifetime distribution from image shown in b with the same colour coding. f Fluorescence decay trace (open black dots), fit (red line), and normalised residual (solid black dots) of a representative pixel (including binning) from image shown in b; IRF = Instrumental Response Function plotted as a blue line. Data shown is representative of 13 cells imaged similarly. Scale bars: 20 µm. Source Data are available as a Source Data file for Fig. 2e, f.
