Fig. 2: Cellular tropism and receptor usage of Ty-BatCoV HKU4 SM3A. | Nature Communications

Fig. 2: Cellular tropism and receptor usage of Ty-BatCoV HKU4 SM3A.

From: Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice

Fig. 2: Cellular tropism and receptor usage of Ty-BatCoV HKU4 SM3A.The alternative text for this image may have been generated using AI.

Ty-BatCoV HKU4 SM3A of 1 MOI was inoculated to a 16 human cell lines; b 12 bat and 10 other mammalian cell lines; c primary Tylonycteris pachypus kidney and lung cells with overexpressed TpDPP4; d different host-DPP4-expressing 293 T cells; e hDPP4 CRISPR-knockout Huh7 cells; and f siRNA hDPP4-knockdown Huh7 cells. Culture supernatants (a–c, e, f) and cell lysates (d) were harvested from respective timepoints and viral titers were determined by RT-qPCR and normalized to β-actin gene. f siRNA efficiency was determined by measuring hDPP4 mRNA expression level in knockdown Huh7 cell lysate at 0 and 24 hpi and compared with mock-treated samples. Data are presented as mean values ± SD, n = 3 independent biological replicates for each cell line at each time point. Dots in each graph represent individual samples. The p-values calculated by multiple two-tailed unpaired t-test without correction for multiple comparisons (<0.05) are indicated in each graph. Statistical significances are indicated by the asterisks (*P < 0.05; **P < 0.01; ***P < 0.0001).

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