Fig. 4: Transgenic mice with hDPP4 expression are permissive to Ty-BatCoV HKU4 infection. | Nature Communications

Fig. 4: Transgenic mice with hDPP4 expression are permissive to Ty-BatCoV HKU4 infection.

From: Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice

Fig. 4: Transgenic mice with hDPP4 expression are permissive to Ty-BatCoV HKU4 infection.The alternative text for this image may have been generated using AI.

a Weight of transgenic mice after challenge with 1 × 106 TCID50 Ty-BatCoV HKU4 SM3A (n = 3) or culture media (n = 3). b RNA levels of Ty-BatCoV HKU4 SM3A detected in lung and brain tissues at 2, 4, 7, 14, and 28 dpi. Data are presented as mean values ± SD, n = 4 (for lung tissues) or 5 (for brain tissues) independent biological replicates at each time point. c IF assay showing antibodies against Ty-BatCoV HKU4 in virus-infected mice serum. Scale bars, 100 μm. d Western blot assay showing antibodies against viral N antigen in virus-infected mice serum. Representative H&E (e) and IHC (f) stained tissue sections from lungs at 4 dpi and brains at 14 dpi. Scale bars, 100 μm. Images are representative of three independent experiments. Uncropped blots in Source Data are provided as Source Data file.

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