Fig. 1: EXTEND Validation. | Nature Communications

Fig. 1: EXTEND Validation.

From: Integrated analysis of telomerase enzymatic activity unravels an association with cancer stemness and proliferation

Fig. 1

a Correlation between EXTEND score and TRAP assay readouts in 28 glioma sphere-forming cell lines. Two ALT cell lines are labeled in blue. For (a–c), Spearman’s correlation was used to calculate P value and Rho, and shade indicates 95% CI of the regression calculated by ggplot2 using default parameters. b Correlation between EXTEND score and direct enzymatic assay results in 11 bladder cancer cell lines. c Correlation between EXTEND score and digital droplet TRAP assay in 15 lung cancer cell lines. d EXTEND scores across ALT and telomerase-positive tumors in liposarcomas (“GSE14533”). Both telomerase-positive tumors (n = 8) and cell lines (n = 8) show significantly higher EXTEND scores than ALT samples (cell lines, n = 8 and tumors, n = 10). e EXTEND scores across the five TMM groups of neuroblastoma (“GSE120572”). Telomerase-positive groups (MYCN amplification (n = 52), TERT high (n = 9), and TERT rearrangement (n = 21)) show significantly higher scores than ALT (n = 31) and no TMM (n = 99) groups. Boxplots, interquartile ranges (25–75th percentile); middle bar defines median and the minima and maxima are within 1.5 times the interquartile range of the lower and higher quartile. Statistical differences were assessed using two-sided Student’s t test in (d) and (e). Data used are available in Source Data.

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