Fig. 2: Rhythmic accumulation of central carbon metabolites in human red blood cells (RBCs).
From: Rhythmic glucose metabolism regulates the redox circadian clockwork in human red blood cells
A Selected significant rhythmic metabolites (*P < 0.05) in human RBCs analyzed by an untargeted metabolomics approach using GC-MS and LC-MS platforms. Rhythmicity analysis was performed using RAIN algorithm (*P < 0.05, FDR = 0.2). RBCs were synchronized for 24 h and then sampling was performed for every 3 h over a period of 48 h. Relative log10 normalized intensities for metabolites are presented. Glycolytic metabolites are represented in blue and PPP metabolites in red. List of identified rhythmic metabolites presented in Supplementary Table 1. Glucose (n = 3 replicates), G6P = glucose-6-phosphate (n = 4 replicates), 2,3-DPG = 2,3-diphosphoglycerate (n = 3 replicates), Ribu5P = ribulose-5-phosphate (n = 4 replicates), R5P = ribose-5-phosphate (n = 3 replicates), pyruvate (n = 3 replicates), lactate (n = 3 replicates). B Correlation plot comparing selected glycolytic and PPP metabolites. Data are mean ± s.e.m.
