Fig. 2: H2AFX expresses both H2A.X poly(A) and SL mRNA isoforms. | Nature Communications

Fig. 2: H2AFX expresses both H2A.X poly(A) and SL mRNA isoforms.

From: Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycle

Fig. 2

a H2A.X mRNA northern blot of total RNA from asynchronous HeLa, HCT-116 and RPE-1 cells. H4 SL: core H4 histone SL mRNA. 18S rRNA used as loading control. b H2A.X mRNA northern blot of total RNA from asynchronous Jurkat cells. 28S and 18S rRNA used as loading controls. c H2A.X mRNA northern blot of total RNA from three different human iPS cell lines. 28S and 18S rRNA used as loading controls. d EdU labelling of asynchronous HeLa, HCT-116, RPE-1 and Jurkat cells showing DAPI fluorescent intensity on x-axis and Alexa 647-EdU fluorescent intensity on y-axis.

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