Fig. 2: PT and IT neurons in ACC are anatomically and functionally distinct.
From: Distinct populations of cortical pyramidal neurons mediate drug reward and aversion
a Left: Viral strategy for anatomical tracing of IT and PT neurons with CTB (N = 3). Right: Quantification of neuronal labeling. Thirty-eight percent of labeled cortical neurons are PT+ (green), ~52% are IT+ (magenta), and ~10% are co-labeled from 86 total cells counted. b Representative photomicrographs of neuronal labeling of IT cells (top), PT cells (middle), and a merged image (bottom). Scale bar = 50 μm. c Line graphs where data points represent the mean ± SEM percent of control (vehicle DREADD, N = 11–12) ambulations induced by a compound stimulus (novelty + 15 mg/kg cocaine, i.p.). IT inhibition (magenta, N = 14) significantly increased and PT inhibition (green, N = 11) significantly decreased the percent of control activity, indicating that the cell types are functionally distinct. **p < 0.001, group × time interaction, two-way RM ANOVA.
