Fig. 3: Neural network classifier suggests cross-inhibited genes are selectively expressed in single cells after co-stimulation. | Nature Communications

Fig. 3: Neural network classifier suggests cross-inhibited genes are selectively expressed in single cells after co-stimulation.

From: Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells

Fig. 3

a UMAP visualization of co-stimulated single cells, colored by the expression score of LPS+IFN-γ (left), or IL-4 (right) UCGs. b Schematic of the neural network classifiers built to categorize cells as LPS+IFN-γ-dominant, IL-4-dominant or Mixed. c Results of the neural network classification of the transcriptional state of co-stimulated cells. Bars represent the number of cells identified as LPS+IFN-γ-dominant (yellow); IL-4-dominant (blue); mixed (green); or unclassified (gray). The classifiers were trained on either all genes or UCGs that displayed cross-inhibition after co-stimulation. Source data are provided as a Source Data file.

Back to article page