Fig. 5: Electron transport inhibits biofilm formation and related gene expression under physiological conditions.

a Representative 3D-reconstructed confocal images of biofilms. The length and width were both 800 nm. b Calculated florescence intensity from 3D-reconstructed confocal images of the biofilms. The biofilms were double-stained with SYTO9 and PI. c PIA contents of the biofilms. The biofilm inducers glucose (G), ethanol (E), and NaCl (N) were added to the TSB medium to final concentrations of 0.5%, 4%, and 2%, respectively. The concentration of NR-MoS2 (NR) was 2 μg mL−1. Data represent the mean ± SD (n = 3 biologically independent samples). Significance was assessed using a two-sided Student’s t-test with multiple comparisons: *p < 0.05. d Schematic diagram showing the NR-MoS2-induced inhibition of biofilm formation under physiological conditions. Source data are provided as a Source Data file.