Fig. 2: Nuclei are mispositioned during cross-species co-infections.
From: Viral speciation through subcellular genetic isolation and virogenesis incompatibility
a–c Cells expressing both GFP-ΦPA3shell (green) and mCherry-ΦKZshell (red) were infected with either ΦPA3 or ΦKZ or both. a ΦPA3 nucleus position when singly infected with ΦPA3 (open circles, n = 100), when dual infected with ΦPA3 (solid squares, n = 56) resulting in two ΦPA3 nuclei in a single cell, or when co-infected with ΦKZ (solid triangles, n = 89). b ΦKZ nucleus position when singly infected with ΦKZ (open circles, n = 100), when dual infected with ΦKZ (solid squares, n = 40) resulting in two ΦKZ nuclei in a single cell, or when co-infected with ΦPA3 (solid triangles, n = 89). c Time-lapse (sec, seconds) of co-infecting nuclei failing to migrate to midcell (mpi, minutes post-infection). d–f Cells expressing wildtype sfGFP-ΦPA3PhuZ (d), mutant sfGFP-ΦPA3PhuZD190A (e), or wildtype sfGFP-ΦKZPhuZ (f) showing the nucleus (blue, DAPI) and spindle (green, GFP), cell membrane (red, FM4-64) when infected with ΦPA3. g, h ΦPA3 nucleus position displayed as fraction of cell length for cells expressing wildtype sfGFP-ΦPA3PhuZ (g, h, green, n = 100), mutant sfGFP-ΦPA3PhuZD190A (g, purple, n = 100), or wildtype sfGFP-ΦKZPhuZ (h, pink, n = 100). Scale bar in panel (d) equals 1 μm and all panels in (c–f) are at the same scale. Data for ΦPA3 nucleus position is repeated in graphs (a, g, & h) for reference. Source data are provided as a Source Data file.
