Table 2 Table of plasmids and strains used in this study.
From: Viral speciation through subcellular genetic isolation and virogenesis incompatibility
Insert | Backbone | Host | Plasmid | Strain | Experiment |
|---|---|---|---|---|---|
sfGFP-ΦPA3shell-mCh-ΦKZshell | pHERD-30T | PA01-K2733 | pMAC082 | MAC084 | Single/dual infection |
sfGFP-ΦPA3PhuZ | pHERD-30T | PA01-K2733 | pVC028 | VC568 | Single infection |
sfGFP-ΦPA3PhuZ(D190A) | pHERD-30T | PA01-K2733 | pVC032 | VC570 | Single infection |
sfGFP-ΦKZPhuZ | pHERD-30T | PA01-K2733 | pVC029 | VC586 | Cross infection |
sfGFP-ΦKZPhuZ(D204A) | pHERD-30T | PA01-K2733 | pVC034 | VC590 | Cross infection |
Untagged ΦKZPhuZ | pHERD-30T | PA01-K2733 | pVC121 | VC614 | Cross infection |
mCh-ΦKZPhuZ-sfGFP-ΦPA3PhuZ | pHERD-30T | PA01-K2733 | pVC120 | VC609 | Single infection |
GFPmut1 | pHERD-30T | PA01-K2733 | pKN053 | KN381 | Single infection |
sfGFP | pHERD-30T | PA01-K2733 | pKN069 | KN529 | Single infection |
ΦPA3gp210-GFPmut1 | pHERD-30T | PA01-K2733 | pMAC185 | EB003 | Cross infection |
ΦPA3gp210-sfGFP | pHERD-30T | PA01-K2733 | pEB001 | EB007 | Cross infection |