Fig. 1: DME and ROS1 act semi-redundantly to promote pollen tube progression.

a Transmission frequency of the dme and ros1 alleles in crosses between Col-0 wild-type mothers and ros1/+, dme/+, dme/+;ros1/+, and dme/+;ros1 pollen donors as bar plot giving the mean ± SD as error bars and individual data points shown as gray dots. Number of individual plants and biological replicates is given below the graph and pairwise comparisons of dme transmission rate between the different crosses revealed significant differences (p-values of two-sided T-tests in pairwise comparison is shown in the graph). b Fluorescent microscopy of aniline blue stained pistils 24 h after crosses of wild-type Col-0 mothers with Col-0, dme/+ and dme/+;ros1 fathers, respectively, revealing pollen tubes in the transmitting tract. Red channel shows autofluorescence of maternal tissues, including the ovules. White arrow indicates aberrant pollen tube trajectory in the mutants; white scale bar equals 20 µm in all photographs. c Violin plots showing the distribution of the transmission frequency of the dme allele in individual siliques from crosses between wild-type Col-0 mother plants and dme/+, as well as dme/+;ros1 fathers with either excess of pollen or excess of ovules (few pollen grains used in the respective cross). Inserted boxplots represent 50% of all values with the second and third quartile separated by the median value as black line, mean value is shown as a red rhomb together with the number (in red). Number of individual plants and siliques (which equals replicates) as well as p-value of two-sided T-test is given. Source data underlying Fig. 1a, c are provided as a Source Data file.