Fig. 2: DME and ROS1 are responsible for DNA demethylation of the vegetative cell in pollen.

a Number and association with genome annotations of CG-DMRs detected in the comparison between VCs and SCs in wild-type (Col), ros1, dme/+ and dme/+;ros1 mutant plants (hypomethylated = lower DNA methylation level in VCs). b Venn diagram showing the overlap of CG-hypo DMRs detected between the VCs and the SCs of Col-0 wild-type, dme/+, and dme/+;ros1. c Heatmap of absolute CG methylation level in SCs and VCs of CG-hypo DMRs detected between VCs and SCs in Col-0 wild type (11,107 regions). The lines represent the indicated genotype for SCs and VCs, respectively. DMRs in the columns were sorted by complete linkage hierarchical clustering using Euclidean distance.