Fig. 3: DME and ROS1 are required for the expression of genes involved in pollen tube function. | Nature Communications

Fig. 3: DME and ROS1 are required for the expression of genes involved in pollen tube function.

From: Male fertility in Arabidopsis requires active DNA demethylation of genes that control pollen tube function

Fig. 3

a Venn diagram giving the number and overlap of genes implied in pollen tube function (see Supplementary Data 9) associated with hyper DMRs in the respective mutant VCs compared to wild-type VCs. b Genome-browser view of two genes involved in pollen tube function (PRK4/AT3G20190 and PLOU/AT2G16030), showing the absolute DNA methylation level for each cytosine context as indicated in the graph for five samples (wild-type [Col-WT] seedlings and VCs of Col-0 wild type, ros1, dme/+ and dme/+;ros1). c RT-qPCR from pollen of the given genotypes of transcripts showing lower abundance in dme/+ and dme/+;ros1 compared to wild-type (Col-0) pollen. For each sample four biological replicates have been used and relative expression level calculated with respect to the reference genes shown on the left. Bars represent mean values with SD shown as error bars. p-values of two-sided T-test in the respective comparison with wild-type level are indicated by stars above the bars; absence of stars indicates no significant difference (p > 0.05; see Supplementary Data 10 for exact p-values). Source data underlying Fig. 3c are provided as a Source Data file.

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