Fig. 4: PRC1-mediated H2AK121ub and EMF1 in collaboration with PRC2 activity are required to reduce chromatin accessibility.

a Accessibility profiles around the TSS of only-H2AK121ub-marked genes in the different mutants. Genes were classified into different groups depending on their levels of H2AK121ub marks relative to WT. The prolife of the different groups is indicated in a color increasing palette form lowest to highest H2AK121ub levels. b Accessibility profiles around the TSS of H2AK121ub/H3K27me3-marked genes in the different mutants, grouping the genes according to their levels of H2AK121ub marks (upper panels) or H3K27me3 (bottom panels) relative to WT. The prolife of the different gene groups is indicated in a color increasing palette form lowest to highest relative H2AK121ub or H3K27me3 levels. c Accessibility profiles around the TSS of only-H3K27me3-marked genes in the different mutants, grouping the genes according to their levels of H3K27me3 marks relative to WT. The prolife of the different gene groups is indicated in a color increasing palette form lowest to highest relative H3K27me3 levels. Gene groups with a very small number of genes were excluded as they produced noisy profiles (see Supplementary Data 3). In all cases (a–c), p values of significant differences between the profile of genes with WT-like levels (80–120%) and those of genes with different levels are indicated according to one-sided Mann–Whitney–Wilcoxon test.