Fig. 3: Active compounds on multiple stages of Plasmodium development after dose–response evaluation and cytotoxicity filtering. | Nature Communications

Fig. 3: Active compounds on multiple stages of Plasmodium development after dose–response evaluation and cytotoxicity filtering.

From: Multistage and transmission-blocking targeted antimalarials discovered from the open-source MMV Pandemic Response Box

Fig. 3: Active compounds on multiple stages of Plasmodium development after dose–response evaluation and cytotoxicity filtering.

a Venn diagram of the number of compounds identified with activity (inhibitory concentration, IC50) below 2 µM, for which no cytotoxicity was identified on either CHO cells (>50% viability at 2 µM or selectivity index >10) or HepG2 cells (selectivity index >10). b A total of two compounds with activity against all life cycle stages tested: Birinapant and AZD-0159. Asexual blood stage activity (ABS) was determined against both drug-sensitive (NF54) and drug-resistant (Dd2) P. falciparum. GC P. falciparum stage IV/V gametocytes. Toxicity indicated both at CC50 (cytotoxic concentration) against HepG2 cells as well as for viability of CHO cells remaining after 20 µM treatment. Data are from three independent biological repeats, performed with minimum technical duplicates, mean ± S.E. 95% confidence intervals on all IC50 values are provided in Supplementary Data 2.

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